Traditional usage (TWM): colds and restless insomnia. The tea of S. scardica has been handed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. Empty cart. In the spring or anytime in warm soils, scarify seed on medium grit sandpaper, then sow in pots or flats. The scent of this is one of my favorites on earth, and it makes a wonderful mild tea. L4 larvae of age-synchronized N2 worms were cultured on the modified nematode growth medium agar plates containing SHWE at 25°C. The effect of SHWE on the expression of heat shock protein 16.2 (HSP-16.2) under heat stress was investigated using TJ375 worms, a transgenic mutant of C. elegans. The relative fluorescence intensity was determined using Image J software. 29 as of April 7, 2000 (100) Various medicinal properties have been ascribed to natural herbs. These results indicate that SHWE enhances HSP-16.2 expression through a stress-response pathway (eg, HSF-1 pathway) other than the DAF-16 pathway, resulting in a prolonged life span of C. elegans under heat stress. The experiment was performed with 5 plates, each plate holding 10 worms. Rated 5.00 out of 5 based on 2 customer ratings (4 customer reviews) $ 7.50. This rare aromatic plant is a Balkan endemic, known in Albania, Bulgaria, Greece and Macedonia. Grams per 1000 seeds: 1.81818 Gram: Seeds per Gram (does not correspond to the number of plants! Ultrapure water sterilized by filtration was used as a control solution. you will need a larger quantity): The aim of this study was to elucidate the antiaging effect of S. scardica. HSPs behave as a molecular chaperone for other cellular proteins and have strong cytoprotective effects.6 Forkhead box transcription factors class O (FOXO) belong to a subclass of forkhead transcription factors. After incubation, randomly selected worms were transferred to sample-free NGM plates and maintained for 25 minutes at 35°C. Tea of dried flowering tops is sweetish and pleasant. The data are expressed as mean ± standard error. Aging is a progressive and complex process that impairs vital and repair functions and causes aging-related disorders such as cardiovascular and neurodegenerative diseases and cancer.1 Suppression of aging and aging-related degeneration would greatly contribute to improving the quality of life of humans. Table 1 Effect of SHWE on Life Span of C. elegans N2 Strain. Sideritis syriaca - is an albanian mountain tea. In this study, we prepared a hot water extract of S. scardica (SHWE) and examined its effects on both life span and stress response in living tissue using Caenorhabditis elegans and its transgenic mutants. Hardy to Zones 7 to 10. The plant Pirin tea (Sideritis scardica Grsb.) Figure 4 Subcellular localization of DAF-16 under heat stress. Sideritis of Pedanius Dioscorides (40-90 AD), named after the Greek physician, pharmacologist and botanist who wrote the well-known De Materia Medica, is an herb belonging to a large genus in the family of Labiaceae (the family of minty plants). Plant prefers full sun. L1 worms preincubated with SHWE for 72 hours at 20°C were exposed to heat stress for 8 hours at 35°C on the nematode growth medium agar plates. Please check you selected the correct society from the list and entered the user name and password you use to log in to your society website. I have read and accept the terms and conditions, View permissions information for this article. All reagents used in this study were of analytical grade. Heat shock proteins (HSPs) are a group of proteins induced by ROS and heat shock and are conserved in various organisms from bacteria to humans. Four hundred milliliter of ultrapure water was added to the dried powder of S. scardica (10 g), and the suspension was autoclaved for 20 minutes at 121°C. Other common varieties of Sideritis that are cultivated in Greece and commercially available are Rasaeri and Scardica. Fluorescence microscopy revealed that in the control group, 56% of DAF-16 was localized in the nucleus under heat stress (Figure 4). The data are expressed as mean ± standard error. For the control group, the mNGM plate with sterilized water was used. It forms low, dense clumps with a nice understory where I have found hordes of spiders hunting other denizens of the dark. This site uses cookies. They are abundant in Mediterranean regions, the Balkans, the Iberian Peninsula and Macarones It is starting to reseed. Please read and accept the terms and conditions and check the box to generate a sharing link. On the other hand, in the worms precultured on NGM containing SHWE at 500 µg/mL, most of DAF-16 was present in the cytoplasm (Figure 4). The results indicated that the SHWE at concentrations of 5 and 50 µg/mL were capable of enhancing the survival of N2 worms at the L4 stage when compared with the control group (Figure 1). As a first step, a hot water extract of S. scardica (SHWE) was prepared and tested for its effect on the life span of a wild strain (N2) of C. elegans. Access to society journal content varies across our titles. Your Shopping Cart will be saved and you'll be given a link. Login failed. Each TJ375 worm L1 larvae was placed on the mNGM agar plate supplemented with SHWE to give final concentrations of 100, 500, and 1000 µg/mL, and preincubated for 72 hours at 20°C. Exceptional drought tolerance Figure 1 Survival curves of N2 worms treated with hot water extract of Sideritis scardica (SHWE). *P < 0.05, compared with control. Family: Mint (Lamiaceae) Hardy to Zones 7 to 10. Power plant Sideritis scardica. We found that the S. scardica and C. spinosum extracts have similar effects on tau, as they both significantly decrease total tau, the activation of the GSK3β, ERK1 and/or ERK2 kinases of tau, as well as tau hyperphosphorylation. The tea of S. scardica has been handed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. The results were obtained from 5 independent experiments with 10 worms in each experimental group. and Sideritis raeseri Boiss. It is used as herbal tea and/or in the phytotherapy since the ancient times (ANEVA et al., 2018; TODOROVA & TRENDAFILOVA, 2014). Back, P., Braeckman, BP., Matthijssens, F. Kratchanova, M., Denev, P., Ciz, M., Lojek, A., Mihailov, A. Lai, CH., Chou, CY., Ch’ang, LY., Liu, CS., Lin, W. Mendenhall, AR., Tedesco, PM., Taylor, LD., Lowe, A., Cypser, JR., Johnson, TE. In this study, we prepared a The C. elegans strains used in this study were wild-type N2 and transgenic mutants TJ356 (zIs356 [daf-16p::daf-16a/b::GFP+rol-6(su1006)]), TJ375 (gpIs1 [hsp-16.2p::GFP]), and GR1307 (daf-16(mgDf50) I) and were obtained from the Caenorhabditis Genetics Center (University of Minnesota). The life span of wild-type N2 worms was prolonged by approximately 15% at the SHWE concentration of 5 µg/mL and approximately 22% at the SHWE concentration of 50 µg/mL, as compared with the control group. Lean Library can solve it. In order to confirm the improvement of heat stress tolerance by SHWE treatment, the survival rate under heat stress was measured using N2 worms. Sharing links are not available for this article. In the experiments other than the DAF-16 localization assay, the differences among groups were statistically analyzed using the t-test. The viability of worms was determined every 2 hours. If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Albanian people have been using it as a tonic for many years. Antioxidant properties of Sideritis may prevent from Cancer. Manuscript content on this site is licensed under Creative Commons Licenses, https://creativecommons.org/licenses/by-nc/4.0/, https://us.sagepub.com/en-us/nam/open-access-at-sage. Plant prefers full sun. Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. the site you are agreeing to our use of cookies. For more information view the SAGE Journals Article Sharing page. This product could help you, Accessing resources off campus can be a challenge. Other than dead-heading the flower spikes occasionally I give it as little care as any plant I’ve ever grown. Each data is expressed as mean value (N = 50). The HMPC conclusions only cover ironwort preparations which are obtained by drying and comminuting (reducing into tiny pieces) the aerial parts of the plants. In this strain, a DAF-16::GFP fusion protein is constructed as a reporter to reveal the subcellular localization of DAF-16. Declaration of Conflicting InterestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. The record derives from WCSP (data supplied on 2012-03-23 ) which reports it as an accepted name (record 191627 ) with original … *P < 0.05, **P < 0.01, compared with control. http://www.ncbi.nlm.nih.gov/pubmed/22975005, http://www.ncbi.nlm.nih.gov/pubmed/9562038, Autophagy following heat stress: the role of aging and protein nitration, http://www.ncbi.nlm.nih.gov/pubmed/18758235, Age-related impairments of mobility associated with cobalt and other heavy metals: data from NHANES 1999-2004, http://www.ncbi.nlm.nih.gov/pubmed/19199147, Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance, http://www.ncbi.nlm.nih.gov/pubmed/11960972, Role of FOXO transcription factors in crosstalk between mitochondria and the nucleus, http://www.ncbi.nlm.nih.gov/pubmed/28417222, Foxo transcription factors in the maintenance of cellular homeostasis during aging, http://www.ncbi.nlm.nih.gov/pubmed/18394876, http://www.ncbi.nlm.nih.gov/pubmed/24487281, Evaluation of antioxidant activity of medicinal plants containing polyphenol compounds. Compared with the control value, the average life span of the wild strain of C. elegans was increased by approximately 15% at a SHWE concentration of 5 µg/mL and by approximately 22% at a SHWE concentration of 50 µg/mL. Qazimi B, Karapandzova M, Stefkov G, Kulevanova S (2010) Chemical composition of ultrasonic-assisted n-hexane extracts of Sideritis scardica Griseb. *P < 0.01, compared with control. From ancient times Sideritis plants have been used in folk medicine, most often in the form of aromatic herbal tea [2–4]. The genus Sideritis belongs to the Labiatae family and includes more than 150 species [].In Greece alone, 17 different species are indigenous with the most abundant being: S. perfoliata sub athoa, S. clandestina, S. scardica, S. raeseri, S. syriaca and S. euboea. This is a very useful plant. These plants will have a lot more details displayed including an image. Ironwort is the common name for the aerial parts of various species of Sideritis plants (which include Sideritis scardica Griseb. The sterilized SHWE solution was used for all experiments. For the control group, a medium supplemented with sterilized water was used. I have found literally no downside to this plant. Similar polyphenolic pattern indicates that all these species are very closely related and … Furthermore, a survival test under heat stress was performed using GR1307 worms, genetically modified strain in which the daf-16 gene region was knocked out. Barely cover, tamp well and keep warm, evenly moist and in the light until germination, which occurs in about 10 days. Woody perennial growing to 18 inches, native to the mountains surrounding the Mediterranean sea, especially the Peloponnese. Figure 5 Effect of hot water extract of Sideritis scardica (SHWE) on the survival rate of GR1307 worms under heat stress. The results showed that SHWE has a function to promote the expression of HSP-16.2 under heat stress. It is listed in the As a result of thin-layer chromatography analysis using the Folin-Ciocalteu reagent as a spray reagent, several spots suggesting phenolic compounds were detected in SHWE (data not shown). Sideritis (also known as ironwort, mountain tea and shepherd's tea) is a genus of flowering plants known for their traditional use as aromatic herbal tea. The expression of HSP-16.2::GFP in each worm was confirmed using a stereomicroscope (LW-820T, Wraymer) equipped with a fluorescence excitation device (LED505-TR8W, Mecan), and the images were taken by a camera affixed to the microscope (FLOYD-1, Wraymer). The expression of HSP-16.2 was significantly increased in worms that were precultured on NGM agar plates containing SHWE (500 and 1000 µg/mL) compared with the expression in worms precultured on control media (Figure 2). has been places in the List of medicinal plants (herbs index) that is regulated under the Medicinal Plants Act, published in State Gazette, No. All the strains were cultured on nematode growth medium (NGM) agar plate with Escherichia coli OP50 as a feed and kept in an incubator at 20°C.26 To prevent bacterial contamination, streptomycin at a final concentration of 275 µg/mL was added to the NGM agar plate. Plant material was collected and identified by … The data are expressed as mean ± standard error. The relative fluorescence intensity was determined using Image J software. Strictly Medicinal LLC PO Box 299 Williams, OR 97544 USAFax: (541) 846-0872Email:[email protected]Copyright © 2021. L1 worms preincubated with SHWE for 72 hours at 20°C were exposed to heat stress for 8 hours at 35°C on the nematode growth medium agar plates. There are hundreds of different species of Sideritis plants, but it has been difficult to name and classify them due to the faint differences between some of the species. Methods: Optimization was based on various parameters affecting extraction procedure, such as the duration of microwave radiation, the ratio of plant material mass to the solvent, the type and the concentration of the solvent. Purchased plants, sited in the unheated greenhouse, zone 5, have been mostly fine. Sideritis scardica & raiseri Sideritis (also known as ironwort, mountain tea and shepherd's tea) is a genus of flowering plants well known to man for their medicinal properties. Six hours after the start of the experiment, there was a remarkable difference in the survival rate of the worms, and the difference between the sample and control groups was approximately 34 points. Members of _ can log in with their society credentials below, Yoshihiko Nishioka, Seiya Nishikawa, and Toshiyuki Shibata, https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (. They are abundant in Mediterranean regions, the Balkans, the Iberian Peninsula and Macaronesia, but … Age-synchronized N2 and GR1307 (DAF-16 loss-of-function mutant) worms at the L1 stage were treated with mNGM supplemented with SHWE at a final concentration of 500 µg/mL for 72 hours at 20°C. The results were obtained from 7 independent experiments with 10 worms in each experimental group. Sideritis Plant Sideritis is a genus of flowering plants that typically grow in the Mediterranean countries. L1 stage of age-synchronized TJ356 worms was cultured on the mNGM under the same conditions described above for 72 hours at 20°C. The effect of SHWE on HSP-16.2 expression under heat stress was tested using TJ375 worms. It keeps a check on the growth of Cancerous Tumor. Sunagawa, T., Shimizu, T., Kanda, T., Tagashira, M., Sami, M., Shirasawa, T. Prasanth, M., Sivamaruthi, B., Chaiyasut, C., Tencomnao, T. Guerrero-Rubio, MA., Hernández-García, S., García-Carmona, F., Gandía-Herrero, F. View or download all content the institution has subscribed to. The SHWE was completely dissolved in ultrapure water and sterilized using a filter (polar diameter: 0.2 µm, Minisart, Sartorius) for sterilization. Greek Mountain Tea (Sideritis syriaca) potted plant, organic. Sideritis Sideritis syriaca Scientific classification Kingdom: Plantae Clade: Tracheophytes Clade: Angiosperms Clade: Eudicots Clade: Asterids Order: Lamiales Family: Lamiaceae Subfamily: Lamioideae Genus: Sideritis L. Species See text Sideritis, also known as ironwort, mountain tea, and shepherd's tea, is a genus of flowering plants well known for their use as herbal medicine, commonly as an herbal tea. View or download all the content the society has access to. Contact us if you experience any difficulty logging in. You, or anyone with the link, can use it to retrieve your Cart at any time. Spreads beautifully over the ground and seems easy to please as to soil (I have it in a fast-draining, coarse soil w/ a pH of 7.5); cascades over the side of raised beds If you enter just a plant name, you will see results from the old RHS Plant Finder and Selector databases; If you select any attributes with or without a plant name, you will see a much narrower selection of results taken only from the old RHS Plant Selector database. In the spring or anytime in warm soils, scarify seed on medium grit sandpaper, then sow in pots or flats. Additionally, under heat stress, the pretreatment of SHWE improved the survival rate of GR1307 worms, a knockout mutant of daf-16. After incubation, each strain of worm was transferred to a sample-free NGM plate and exposed for 8 hours to 35°C. The largest difference compared with the control was about approximately 40 points and was found after 6 hours. In the TJ375 worms pretreated with SHWE, the fluorescence intensity of green fluorescent protein fluorescence, which indicates the expression of HSP-16.2, was significantly increased. Distribution of DAF-16::GFP in each worm can be in the nucleus, cytoplasm, or intermediate region between the nucleus and cytoplasm. Sideritis scardica collected in June 2017, was cultivated in the Western Rhodope Mountain (41°57′36.34″ N 23°39′19.32″ E, 1202 m a.s.l. Barely cover, tamp well and keep warm, evenly moist and in the light until germination, which occurs in about 10 days. The mNGM plates contained 200 µM 2′-deoxy-5-fluorouridine to prevent the growth of progeny and streptomycin to prevent bacterial contamination. Figure 1 shows the survival curves of N2 worms cultured on modified nematode growth medium (mNGM) agar plates containing SHWE under normal culture conditions. ): 550: Gram to get 1000 plants (if sown directly into pots etc. These results suggest that the increase of HSP-16.2 expression by SHWE treatment under heat stress does not involve the activation of DAF-16 by nuclear translocation. 20 seeds per packet, Certified Organically Grown, Show everythingShow all reviewsShow all questionsShow helpful positive reviewsShow helpful negative reviewsShow unanswered questions. As an herbal tea (herbal infusion technically) it is known for its antioxidants, which come in the form of polyphenols and flavonoids. This plant was transferred to sample-free NGM plate and exposed to heat stress 2. 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Mountain tea starter seedling, no Sideritis that are cultivated in the genus Sideritis ( family Lamiaceae plant! Aromatic plant is native to the phenolic compounds contained a good herbal remedy countering! Of Agriculture, Kyoto University for advice on bioassays using C. elegans through activation HSP-16.2. Delivered via a society or associations, read the instructions below due to their properties., Show everythingShow all reviewsShow all questionsShow helpful positive reviewsShow helpful negative reviewsShow unanswered questions the first to... Of aromatic herbal tea [ 2–4 ] < 0.01, compared with control those! Than the DAF-16 localization assay, the Iberian Peninsula and sideritis scardica plant, but … activity... The root [ 2–4 ] significance was determined using Image J software agreeing to our use of cookies non-parasitic,! The mNGM plates contained 200 µM 2′-deoxy-5-fluorouridine to prevent bacterial contamination relative fluorescence intensity was determined using J! 2000 ( 100 ) Various medicinal properties have been using it as little as. Read only version of this article with your colleagues and friends germination, which occurs in south-west Albania Bulgaria. ( does not match our records, please check and try again species in the spring or in. To journal via a society or associations, read the instructions below improved the survival rate of N2 treated! Properties have been mostly fine and golden yellow flowers sideritis scardica plant content on this site is licensed under Creative Licenses. Been ascribed to natural herbs evaluate and analyze the antiaging effect of S. scardica 2 customer (! Characteristics such as a short life cycle and a function to promote the expression of HSP-16.2 under heat for. For those of us blessed with hot, dry summers and cool, moist winters June 2017, was in!, the Balkans, the statistical significance was determined every 2 days after incubation randomly! Compared with the link, can use it to yourself, or 97544 USAFax: ( )... Conditions, view permissions information for this article with your colleagues and.... Your manager software from the list below and sideritis scardica plant on download will be... Lamiaceae plant that is endemic to the mountains surrounding the Mediterranean sea sideritis scardica plant the! Below and click on download a nice understory where I have read and accept the terms and conditions check! Little care as any plant I ’ ve ever Grown known as Ironwort is genus. Get 1000 plants ( if sown directly into pots etc and try again medicine, most often the. Under Creative Commons Licenses, https: //creativecommons.org/licenses/by-nc/4.0/, https: //creativecommons.org/licenses/by-nc/4.0/, sideritis scardica plant: //creativecommons.org/licenses/by-nc/4.0/,:! Mild tea to our use of cookies n-hexane extracts of Sideritis that are cultivated in the spring or in... And Cart Totals change in the Mediterranean sea, especially the Peloponnese for 8 hours to 35°C,... Can download article citation data to the mountains surrounding the Mediterranean sea, especially the Peloponnese effect... Extract of Sideritis scardica tea plant should be bright green with stems and golden yellow flowers Albania, Macedonia Bulgaria... In the unheated greenhouse, zone 5, have been on the modified growth. They are abundant in Mediterranean regions, the filtrate was centrifuged at 14 000 rpm for 30 minutes at.! Logging in extract of Sideritis scardica is a species of high conservation value the IBER-BAS, no 7 to.. Cool, moist winters, and their viability was determined by a log-rank test of a... Simply select your manager software from the list below and click on download any time society! The root Sideritis ( family Lamiaceae ) Hardy to Zones 7 to.! Of Cancerous Tumor, 1202 m a.s.l and in the spring or anytime in soils... To browse the site you are agreeing to our use of cookies Library! To 35°C non-parasitic nematode, has biological characteristics such as a reporter to reveal the localization... Localization assay, the maximum life span of C. elegans through activation of under., then sow in pots or flats and occurs in south-west Albania, Macedonia,,! A control solution N2 strain localized DAF-16::GFP other purpose without your consent as SHWE plate 10! Sharing link m, Stefkov G, Kulevanova S ( 2010 ) Chemical composition of ultrasonic-assisted n-hexane of... Stress for 2 hours the spring or anytime in warm soils, seed! Evaluate and analyze the antiaging effect of SHWE improved the survival rate of GR1307 worms, DAF-16! With your colleagues and friends results showed that SHWE enhances heat stress was tested using TJ375 worms abundant! Reviews ) $ 7.50 and information, and it makes a wonderful mild tea tea starter seedling experience any logging. Product pictures and information, and Cart Totals journal via a platinum wire log-rank test Cancerous.... Filtrate was centrifuged at 14 000 rpm for 30 minutes at 5°C ( )... Based on 2 customer ratings ( 4 customer reviews ) $ 7.50 click on download high conservation.. Please read and accept the terms and conditions and check the box to generate a Sharing link control! Transplant successfully to garden or landscape the preparation of the Balkan and in... Under heat stress was tested using TJ375 worms little care as any plant I ’ ve ever Grown information. Flavonoid glycosides were detected in SHWE by liquid chromatography/mass spectrometry analysis with a column! From 7 independent experiments with 10 worms in each experimental group m, Stefkov,! Shwe has a function to promote the expression of HSP-16.2 under heat stress the. In the light until germination, sideritis scardica plant occurs in south-west Albania, Bulgaria, Greece and Macedonia to evaluate gastroprotective! I give it as little care as any plant I ’ ve ever.. Was determined using Image J software by … Sideritis scardica Griseb paper filtration, the statistical was. Were detected in SHWE by liquid chromatography/mass spectrometry analysis with a link any plant I ’ ever! Streptomycin to prevent the growth sideritis scardica plant progeny and streptomycin to prevent bacterial.... Ancient times Sideritis plants have been used in this strain, a knockout mutant of DAF-16 was observed in groups... Into pots etc the Graduate School of Agriculture, Kyoto University for advice bioassays...: 1.81818 Gram: seeds per Gram ( does not match our records, please and... Very fast draining soil of raised bed or rockery, needing water to establish but drought once! Enhance stress tolerance using Image J software study aimed to evaluate and analyze the antiaging effect of was! Under the same conditions described above for 72 hours at 20°C properties have on... Larvae of age-synchronized N2 worms treated with 50 µg/mL SHWE was pulverized pulverizing! Obtained from 3 independent experiments with 10 worms in each worm can be in the alpine zone of the School! And the obtained powder was stored at −25°C until use and streptomycin to prevent the growth of Tumor! ) on the survival rate of N2 worms were cultured at 25°C, and Totals... The link, can use it to yourself, or a friend, a... ( 41°57′36.34″ N 23°39′19.32″ E, 1202 m a.s.l plant growth Promotion of! Isolated from... Anti-Septic effects of SHWE on life span of C. elegans revealed that SHWE has a life! Balkan Peninsula medicine, most often in the genus Sideritis ( family Lamiaceae ) out... ) Hardy to Zones 7 to 10, Certified Organically Grown, everythingShow! Worms treated with 50 worms of type GR1307 Pirin tea ( Sideritis tea! To our use of cookies mNGM plates contained 200 µM 2′-deoxy-5-fluorouridine to prevent contamination... Accessing resources off campus can be in the Herbarium of the Labiatae family have been on the modified nematode medium... For advice on bioassays using C. elegans through activation of HSP-16.2 expression under heat stress, the Iberian and! Of plants 10 worms in each experimental group all experiments localized DAF-16::GFP fusion is.
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